| Autor: |
Robert Davies, Ernesto Liebana, M. D. Hampton, Emma L. Best, Felicity A. Clifton-Hadley, Steen Ethelberg, John Threlfall |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
International Conference on the Epidemiology and Control of Biological, Chemical and Physical Hazards in Pigs and Pork. |
| Popis: |
In November 2006 a cluster of Salmonella Typhimurium DT120 in the North East of England was putatively associated with the consumption of pork. At the same time cases of illness in Denmark were associated with this Salmonella type, and a EU alert was issued to determine the type of S. Typhimurium DT120 identified. Isolates from the UK and Denmark were compared on the basis of antib1ogram, Pulsed Field Gel Electrophoresis (PFGE) and Multi-Locus-Variable number tandem repeat Analysis (MLVA or VNTR) to identify the S. Typhimurium DT 120 type and results were compared electronically. Isolates from England had the resistance profile ApSSuT (ampicillin, streptomycin, sulfamethoxazole and tetracycline), VNTR profile (171-244-316-0-487) and with the distinct PFGE type (STYMXB.0083). Isolates from Denmark were resistant to Ap (ampicillin) only, had the VNTR type (171-270-324-0-490) and a PFGE type distinct from England (STYMXB.0010). It was therefore possible to confirm that the isolates from England and Denmark were not identical. These results have verified the significance of VNTR in outbreak investigations for S. Typhimurium and have demonstrated how new molecular strategies may be used to supplement existing methods such as PFGE to enable the accurate and rapid comparison of isolates from different countries. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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