Some Observations upon the Golgi Elements of the Anterior Pituitary Cells of Normal and Stilboestrol-Treated Male Rats, using the Sudan Black Technique
| Autor: | C. L. Foster |
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| Rok vydání: | 1947 |
| Předmět: | |
| Zdroj: | Journal of Cell Science. :409-418 |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.s3-88.4.409 |
| Popis: | 1. It was found possible to demonstrate the Golgi zones of the cells of the anterior pituitary (pars distalis) of the rat with Sudan black. The position and general morphology of the Golgi bodies closely resembled that revealed by the standard osmium tetroxide techniques. 2. The Golgi zone was generally found to contain three sorts of element: (a) diffuse sudanophil material in which were embedded: (b) clear vacuoles and (c) strongly sudanophil bodies. The latter were often associated with the surfaces of the vacuoles, when they were in the form of (I) crescentic caps, (2) partial or complete rims of apparently uniform thickness, or (3) small granules. Sometimes the strongly sudanophil bodies were granular and unassociated with vacuoles; there were also vacuoles with no demonstrable lipoid material in association with their surfaces. 3. The effect of stilboestrol was to cause hypertrophy of the Golgi zones and this was associated with (a) an increase in volume of the region of diffuse lipoids, (b) an increase in vacuolation, and (c) a rather more variable increase in the amount of strongly sudanophil material. These changes would appear to indicate a state of heightened secretory activity. 4. The strong affinity of the Golgi area for sudan black suggests that it consists, at any rate in part, of lipoids. |
| Databáze: | OpenAIRE |
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