Gene Therapy for Repair and Proliferation of Human Dental Pulp Stem Cells – An In-Vitro Study

Autor:	Swathi L Nandawadagi, Indiresha H Narayana, Ashmitha Kishan Shetty
Rok vydání:	2022
Zdroj:	ECS Transactions. 107:14719-14727
ISSN:	1938-6737 1938-5862
Popis:	Regulatory cellular process of mesenchymal stem cell remains to be explicated in diseased condition. On the other hand, platelet-derived growth factors (PDGFs) are known to shape the migratory movements of the mesenchymal cells during stressful conditions. The objective of the current study is to understand the role of Recombinant Platelet-Derived Growth Factors-BB (rhPDGF-BB) towards proliferation and migration of Human Dental Pulp Stem Cells (hDPSC). The cytotoxicity of rhPDGF-BB was assessed at various doses and its chemotactic response was measured by experimental wound scratch test. The recombinant protein did not show cytotoxicity at higher doses. It was found that rhPDGF-BB is a strong inducer of indiscriminate as well as directional migration of hDPSCs that persisted upon 24hrs. Thus, the experiment demonstrates critical use of rhPDGF-BB in regenerative process during dental pulp stem injury.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ee7ded40b50b703654e35ac2d8e3f390 https://doi.org/10.1149/10701.14719ecst Zobrazit plný text záznamu