Derivatization of Drugs and Bioorganics for Improved Detection by Gas Chromatography and Photoionization Detection (GC-PID)
| Autor: | I.S. Krull, M. Swartz, J. N. Driscoll |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Response factor
Detection limit Chromatography genetic structures Biochemistry (medical) Clinical Biochemistry Analytical chemistry PID controller Alcohol Photoionization Biochemistry Chloride Analytical Chemistry chemistry.chemical_compound chemistry Electrochemistry medicine Gas chromatography Derivatization Spectroscopy medicine.drug |
| Zdroj: | Analytical Letters. 18:2619-2632 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032718508064491 |
| Popis: | Pentafluorophenyldimethylsilylchloride (flophemesyl chloride, FICI) has recently been used for derivatizations of alcohols, leading to significant improvements in detectability by gas chromatography (GC)-photoionization detection (PID) and electron capture detection (ECD). This off-line reaction has now been applied to a number of related alcohol derivatives, naturally occurring bioorganics such as cholesterol, androsterone, and similar sterols and steroids. Standard GC-ECD-PID analytical parameters have been determined, such as detection limits, calibration plots, ECD and PID relative response factors (RRF), ECD/PID relative response factor ratios, etc. for underivatized and derivatized substrates. Packed and capillary column GC conditions have been used with a 10.2 eV PID lamp. Application of the optimized derivatization-GC-ECD-PID method is reported for bioorganics in biological media. The overall results demonstrate the immediate applicability of such an approach for a number of alcohol deriv... |
| Databáze: | OpenAIRE |
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