Peptide nucleic acid as a selective recognition element for electrochemical determination of Hg2+
| Autor: | Agnieszka Bala, Łukasz Górski |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Peptide nucleic acid
Chemistry Inorganic chemistry Biophysics 02 engineering and technology General Medicine 010402 general chemistry 021001 nanoscience & nanotechnology Electrochemistry 01 natural sciences Redox 0104 chemical sciences Dielectric spectroscopy chemistry.chemical_compound Peptide bond Physical and Theoretical Chemistry Ferrocyanide 0210 nano-technology Biosensor Voltammetry |
| Zdroj: | Bioelectrochemistry. 119:189-195 |
| ISSN: | 1567-5394 |
| DOI: | 10.1016/j.bioelechem.2017.09.008 |
| Popis: | A novel electrochemical PNA-based biosensor for the determination of Hg 2 + is described. The receptor layer, containing single strands of polythymine PNA (peptide nucleic acid), was formed at the surface of gold electrode. Due to the presence of thymine bases and peptide bonds, an interaction between Hg 2 + ion and receptor layer occurs. The influence of chain modification – PNA vs. DNA – and type of redox marker – anionic AQMS-Na (sodium salt of anthraquinone-2-sulfonic acid) and Fe II/III (potassium ferri/ferrocyanide) or cationic MB (methylene blue) and RuHex (hexaammineruthenium(III) chloride) – were studied. Proposed PNA-based biosensor with anionic AQMS-Na as a redox marker demonstrated significantly better analytical parameters, as compared to results obtained for other tested redox markers (for measurements at pH 6.0). The linear response towards Hg 2 + was in the range from 5 to 500 nmol·L − 1 with the detection limit of 4.5 nmol·L − 1 . The developed sensor distinguishes itself with high selectivity towards Hg 2 + , even for solutions containing several interfering cations. Interactions between Hg 2 + and PNA receptor layer were studied using square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS). |
| Databáze: | OpenAIRE |
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