Accurate high throughput quantification of selenium in biological samples – the potential of combining isotope dilution ICP-tandem mass spectrometry with flow injection

Autor:	Nataliya Rohr-Udilova, Luca Bamonti, Sarah Theiner, Gunda Koellensperger, Bernhard K. Keppler
Rok vydání:	2016
Předmět:	Chromatography Tandem Chemistry 010401 analytical chemistry chemistry.chemical_element Isotope dilution 010402 general chemistry Serum samples Tandem mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry Sample preparation Spectroscopy Selenium
Zdroj:	Journal of Analytical Atomic Spectrometry. 31:2227-2232
ISSN:	1364-5544 0267-9477
DOI:	10.1039/c6ja00209a
Popis:	In this work, different strategies for the analysis of selenium in human serum were validated by tandem ICP-MS and isotope dilution. The potential of flow injection–ICP-tandem MS was explored to provide accurate quantification of selenium in serum samples, thus allowing high throughputs and low sample consumption (as low as 10 μL) without the requirement of sample preparation. The approach combining on-line isotope dilution and the use of gas modes (either oxygen or hydrogen) is ideally suited to clinical analysis. As a key prerequisite, the 77Se-enriched spike was added on-line to avoid precipitation effects that would occur if samples rich in proteins are mixed with isotopically enriched standards without undergoing previous mineralization procedures. This method proved to be fit for purpose as being validated by using a Serum-Clinchek-certified reference material and by comparing isotope dilution analysis as performed on mineralized samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ee3427c649c097473dd92793ef38ac2d https://doi.org/10.1039/c6ja00209a Zobrazit plný text záznamu