Fusion of the Saccharomyces cerevisiae leu2 gene to an Escherichia coli beta-galactosidase gene

Autor:	M J Casadaban, A E Martinez-Arias
Rok vydání:	1983
Předmět:	Regulation of gene expression Mutant Saccharomyces cerevisiae Cell Biology Biology medicine.disease_cause biology.organism_classification Molecular biology Yeast Plasmid maintenance Gene product Biochemistry medicine Molecular Biology Escherichia coli Gene
Zdroj:	Molecular and Cellular Biology. 3:580-586
ISSN:	1098-5549 0270-7306
DOI:	10.1128/mcb.3.4.580
Popis:	The promoter and translation initiation region of the Saccharomyces cerevisiae leu2 gene was fused to the Escherichia coli beta-galactosidase gene. This fusion located the control region of the leu gene and orientated its direction of expression. When the fusion was placed into yeast cells, beta-galactosidase was expressed under the same regulatory pattern as the original leu2 gene product: its synthesis was repressed in the presence of leucine and threonine. Sensitive chromogenic substrates for beta-galactosidase were used to detect expression in isolated colonies growing on agar medium. Mutant yeast cells with increased beta-galactosidase activity were identified by the color of the colonies they formed. One class of mutants obtained appeared to affect ars1 plasmid maintenance, and another class appeared to affect beta-galactoside uptake.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ee30fbdada29342edf98d7a908f046ae https://doi.org/10.1128/mcb.3.4.580 Zobrazit plný text záznamu