Biosynthesis of isocitric acid from ethanol by yeasts

Autor:	N. V. Shishkanova, S. S. Eremina, E. A. Fausek, Finogenova Tatyana
Rok vydání:	1991
Předmět:	Ethanol biology Glyoxylate cycle Substrate (chemistry) Isocitric acid General Medicine Applied Microbiology and Biotechnology Enzyme assay Yeast chemistry.chemical_compound Biochemistry chemistry Biosynthesis cardiovascular system biology.protein Citric acid Biotechnology
Zdroj:	Applied Microbiology and Biotechnology. 36:231-235
ISSN:	1432-0614 0175-7598
DOI:	10.1007/bf00164426
Popis:	The ability of yeasts of different taxonomic groups to synthesize isocitric (ICA) and citric acids (CA) was studied during their growth on ethanol medium. The most active producer of ICA from ethanol, Candida lipolytica 704, was selected. We have chosen cultivation conditions, i.e. pH of the medium and concentrations of oxygen and substrate, optimal for accumulation of ICA. The yield of ICA exceeded 60% of the weight of substrate used. We have studied the dynamics of acid production under conditions of periodic growth of C. lipolytica 704 on ethanol. On the basis of the analysis of citrate and glyoxylate cycle enzyme activity we have developed a conception for the mechanism of ICA overproduction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ee18c7051c92845d92e6fd775bb4aac5 https://doi.org/10.1007/bf00164426 Zobrazit plný text záznamu Full text from SpringerLink