A Method for Fixation of Fish Larvae for Morphological and Genetic Studies

Autor:	S. A. Evseenko, S. G. Kobyliansky, N. V. Gordeeva
Rok vydání:	2019
Předmět:	chemistry.chemical_compound Chromatography Chemistry Dimethyl sulfoxide Buffer solution Aquatic Science Commercial kit General Agricultural and Biological Sciences Fish larvae DNA extraction Fixative DNA Fixation (histology)
Zdroj:	Journal of Ichthyology. 59:818-822
ISSN:	1555-6425 0032-9452
DOI:	10.1134/s0032945219050035
Popis:	Three aqueous buffer solutions that make it possible to stabilize DNA and also preserve the initial body shape and morphological structures are tested for fixation and preservation of deep-sea fishes’ larvae. Based on the quality assessment of DNA and appearance of the larvae 6 months after fixation, the buffer solution containing dimethyl sulfoxide is recommended for use. Unlike ethanol or formalin, this fixative is nontoxic, nonflammable, does not require storage of samples in the refrigerator, and is also compatible with standard DNA extraction methods or commercial kit protocols.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ee0d3c5534a09e0cbb362499c5ff94b4 https://doi.org/10.1134/s0032945219050035 Zobrazit plný text záznamu Full text from SpringerLink