Precise Control of the Assembly of Dye-Coded Oligonucleotide Silver Nanoparticle Conjugates with Single Base Mismatch Discrimination Using Surface Enhanced Resonance Raman Scattering
| Autor: | W. Ewen Smith, Duncan Graham, David G. Thompson, Karen Faulds |
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| Rok vydání: | 2009 |
| Předmět: |
Nanostructure
Oligonucleotide Chemistry Nanoparticle Nanotechnology Combinatorial chemistry Silver nanoparticle Surfaces Coatings and Films Electronic Optical and Magnetic Materials symbols.namesake General Energy Colloidal gold symbols Physical and Theoretical Chemistry Raman spectroscopy Raman scattering Conjugate |
| Zdroj: | The Journal of Physical Chemistry C. 114:7384-7389 |
| ISSN: | 1932-7455 1932-7447 |
| Popis: | The control of the assembly of nanostructures of dye-coded oligonucleotide−silver nanoparticle (DOSN) conjugates (silver nanoparticles cofunctionalized with thiol modified DNA and a Raman reporter molecule) in a sandwich assay format with single base discrimination in an unmodified target oligonucleotide is reported. Rational placement of a single base mismatch in the 18 base duplex formed between target and DOSN conjugates completely controls the hybridization event, preventing or allowing nanostructure formation. The assembly process was monitored using UV−vis spectrometry and surface-enhanced resonance Raman scattering (SERRS). These two supporting analysis techniques show that there is an initial formation of small, highly SERRS active assemblies followed by the formation of larger superaggregates. The rapid increase in SERRS intensity obtained from DOSN conjugate hybridization has been utilized for the near-immediate discrimination of a single base mismatch using SERRS. This report highlights the exq... |
| Databáze: | OpenAIRE |
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