The effect of titanium dioxide nanoparticles on antioxidant gene expression in tilapia (Oreochromis niloticus)
| Autor: | Ruth Varela-Valencia, Omar Zapata-Pérez, Nikte Gómez-Ortiz, Gerko Oskam, Romeo de Coss, Jorge Rubio-Piña, Marcela Del Río-García, Arnulfo Albores-Medina |
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| Rok vydání: | 2014 |
| Předmět: |
Anatase
food.ingredient Materials science Bioengineering Nanotechnology Superoxide dismutase chemistry.chemical_compound food Gene expression General Materials Science chemistry.chemical_classification Reactive oxygen species biology technology industry and agriculture Tilapia General Chemistry Condensed Matter Physics biology.organism_classification Molecular biology Atomic and Molecular Physics and Optics Oreochromis chemistry Catalase Modeling and Simulation Titanium dioxide biology.protein |
| Zdroj: | Journal of Nanoparticle Research. 16 |
| ISSN: | 1572-896X 1388-0764 |
| DOI: | 10.1007/s11051-014-2369-3 |
| Popis: | The reactivity of nanoparticles (NPs) in biological systems is well recognized, but there are huge gaps in our understanding of NP toxicity in fish, despite a number of recent ecotoxicity studies. Therefore, the aim of this research was to evaluate the effect of titanium dioxide NPs (TiO2-NPs) on antioxidant gene expression in the tilapia, Oreochromis niloticus. First, different sizes, shapes, and phases of TiO2-NPs were synthesized and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and dynamic light scattering (DLS). Fish were injected intraperitoneally with different concentrations (0.1, 1.0, 10.0 mg/L), sizes (7, 14, and 21 nm), and phases (anatase and rutile) of TiO2-NPs, and sacrificed 3, 6, 12, and 24 h after injection, when their livers were removed. Total RNA was extracted, and expression of the catalase (CAT), glutathione-S-transferase (GST), and superoxide dismutase (SOD) genes was assessed by real-time polymerase chain reaction (RT-PCR). The results showed that injection of 1.0 mg/L TiO2-NPs induced an initial mild increase in CAT, GST, and SOD gene expression in tilapia, after which transcript levels decreased. Fish injected with 7 and 14 nm TiO2-NPs showed an increase in antioxidant transcript levels 6 h after treatment. Finally, the rutile form generated stronger induction of the GST gene than anatase TiO2-NPs during the first 6 h after injection, which suggests that exposure to rutile causes higher levels of reactive oxygen species to be produced. |
| Databáze: | OpenAIRE |
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