Using active microfluidic flow focusing to sort particles and cells based on high-resolution volume measurements
| Autor: | Jason Riordon, Michel Godin, Michael Nash, Matias Calderini |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Resistive touchscreen
education.field_of_study Materials science Microfluidics Population Sorting Nanotechnology Condensed Matter Physics Atomic and Molecular Physics and Optics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Flow focusing Coulter counter sort Sensitivity (control systems) Electrical and Electronic Engineering Biological system education |
| Zdroj: | Microelectronic Engineering. 118:35-40 |
| ISSN: | 0167-9317 |
| DOI: | 10.1016/j.mee.2014.02.003 |
| Popis: | We combine a high-resolution resistive pulse sensor with hydrodynamic flow focusing.We quantify sorting purity, throughput and resolution with polystyrene microspheres.We size/sort a population of Saccharomyces cerevisiae (yeast) cells.We integrate PDMS valves, both for sensitivity enhancement and cell collection. We demonstrate a microfluidic device that integrates high-sensitivity volume sensing with active pressure-driven flow sorting. Label-free size-based sorting of microparticles and cells is achieved using hydrodynamic flow focusing combined with a resistive pulse sensor with tunable sensitivity that utilizes the Coulter principle. Measurements are performed in real time, and pressure-driven flows are automatically adjusted in feedback to direct microparticles into one of multiple outlet sorting channels at 97.3% efficiency and with a resolution of ~3µm3. In a subsequent test, cells were sorted by volume from a single culture of Saccharomyces cerevisiae (yeast) at 100% efficiency. Next, we demonstrate the device's ability to size a population of cells at high-throughput and identify features of interest prior to sorting. Subpopulations of arrested G1 and M-phase cells were successfully resolved from a single yeast culture. This integrated on-chip sizing and sorting method is ideal for sorting small numbers of particles/cells at very high resolution. |
| Databáze: | OpenAIRE |
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