IL-23-producing CD68+ macrophage-like cells predominate within an IL-17-polarized infiltrate in chronic periodontitis lesions

Autor: Jean-Pierre Allam, James Deschner, Friedhelm Heinemann, Natalija Novak, Søren Jepsen, Jochen Winter, Werner Götz, Thomas Bieber, Yonggang Duan, Matthias Wenghoefer
Rok vydání: 2011
Předmět:
Zdroj: Journal of Clinical Periodontology. 38:879-886
ISSN: 0303-6979
1600-051X
DOI: 10.1111/j.1600-051x.2011.01752.x
Popis: Allam JP, Duan Y, Heinemann F, Winter J, Gotz W, Deschner J, Wenghoefer M, Bieber T, Jepsen S, Novak N. IL-23 producing CD68+ macrophage-like cells predominate within a IL-17 polarized infiltrate in chronic periodontitis lesions. J Clin Periodontol 2011; 38: 879–886. 38: 879–886. doi: 10.1111/j.1600-051X.2011.01752.x. Abstract Aim: To analyse antigen-presenting cells (APCs), such as dendritic cells (DCs), macrophages (Mo) or B cells depending on the regional site of chronic periodontitis (CP), and to investigate their relation to Th17 cells. Material and Methods: Biopsies from oral mucosa as well as the coronal and bottom regions of CP were analysed by immunhistochemistry, immunofluorescence, flow cytometry and real-time PCR. Results: A predominance of CD68+ Mo-like cells and CD20+ B cells and strong Th17 infiltration was observed in the bottom region of CP lesions, while CD1a+ DCs were only detected in the coronal regions, where Th17 infiltration was low. Furthermore, CD68+ Mo-like cells displayed CD163 expression as a typical Mo-marker, but expressed in parallel typical DCs markers, such as CD11c or CD209 and TLR4. Interestingly, Th17-inducing cytokine IL-23p19 was produced by CD68+ Mo-like cells, but not CD20+ B cells. Moreover, the stimulation of in vitro generated CD68+ Mo-like cells by Porphyromonas gingivalis-derived (Pg) lipopolysaccharide resulted in the upregulation of their IL-23p19 mRNA expression, which was inhibited by the blockage of TLR4. Conclusions: In view of these data, a picture emerges that IL-17-producing cells in CP could be in part directed by CD68+ Mo-like cells, which produce IL-23p19 upon TLR4 activation by Pg.
Databáze: OpenAIRE
Externí odkaz: