Regrowth of embryogenic tissues of Pinus nigra following cryopreservation

Autor: Beáta Piršelová, Ildikó Matušíková, Lenka Fráterová, Terézia Salaj, Jan Salaj
Rok vydání: 2010
Předmět:
Zdroj: Plant Cell, Tissue and Organ Culture (PCTOC). 106:55-61
ISSN: 1573-5044
0167-6857
DOI: 10.1007/s11240-010-9893-3
Popis: Embryogenic tissues of a conifer species Pinus nigra Arn. have been cryopreserved by a slow-freezing method. The effect of cryoprotective compounds such as maltose, sucrose or sorbitol (each 0.5 M) combined with 7.5% (v/v) DMSO has been tested. After thawing, the following parameters have been investigated: tissue regrowth 6 weeks after thawing, and post-thaw growth evaluated as fresh mass accumulation as well as genetic stability in post-thaw period. The parameters mentioned have been compared in both cryopreserved and non-cryopreserved tissues. Out of eight cell lines used in experiments, seven survived cryopreservation (87.5% regrowth), although cell line and treatment effects were observed. In most cell lines, sucrose or maltose pretreatments were superior over sorbitol. In the regrown cell lines, the post-thaw growth as fresh mass accumulation was not negatively influenced by cryopreservation. No genetic variation was observed in cryopreserved tissues using a RAPD approach.
Databáze: OpenAIRE
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