| Autor: |
Vladimir Bondar, Xiaomin Hu, Lunguang Yao, Li Na, Zhihan Guan, Liu Yangkun, Alexey Puzyr, A. E. Burov, Nannan Zhang |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.21203/rs.3.rs-46171/v1 |
| Popis: |
Background Current inactivated and attenuated vaccines can not provide complete protection, resulting in the ongoing epidemic of porcine epidemic diarrhea virus (PEDV), which has caused significant economic losses in the swine industry worldwide. To develop an alternative efficient and economical vaccine, a fusion protein named S1CD containing multiple neutralizing epitopes of PEDV was designed and expressed in Sf9 cells. The expression yield was optimized by production of baculoviral constructs harboring multiple S1CD-expressing cassettes, and the immunogenicity of the purified protein was evaluated. Results The results showed that high-level expression of the S1CD fusion protein was achieved by multi-copy gene co-expression. The recombinant virus carrying three copies of S1CD gene under the control of p10 promoter showed the highest expression level, with a yield of 462 mg/L, which is about 2.6 or 3.5 fold that of the recombinant viruses carrying only one copy. BALB/c mice were subcutaneously immunized with purified S1CD protein three times at 2 week intervals. After immunization, S1CD protein could induce the mice produce high level of specific IgG antibodies and neutralizing antibodies. Conclusion Fusion S1CD protein consisting of multiple epitopes of porcine epidemic diarrhea virus was highly expressed by multi-copy gene co-expression. Moreover, it stimulated mice to produce high level of specific IgG antibodies and neutralizing antibodies. The S1CD protein expressed in this study could be used as a putative economic and efficient subunit vaccine against PEDV infection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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