Circulating osteopontin released by injured kidneys causes pulmonary inflammation and edema
| Autor: | Fatima Zohra Khamissi, Daniel Kreisel, Liang Ning, Hao Dun, Andreas Herrlich, Amy J. Keller, Sabine Dietmann, Jeffrey J. Atkinson, Brian W. Wong, Akshayakeerthi Arthanarisami, Eirini Kefaloyianni, Wenjun Li, Kory J. Lavine |
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| Rok vydání: | 2021 |
| Předmět: |
Pathology
medicine.medical_specialty Kidney Lung biology urogenital system business.industry Acute kidney injury respiratory system Lung injury urologic and male genital diseases medicine.disease female genital diseases and pregnancy complications respiratory tract diseases Transplantation medicine.anatomical_structure Respiratory failure Edema medicine biology.protein Osteopontin medicine.symptom business |
| DOI: | 10.1101/2021.07.20.452998 |
| Popis: | Multiorgan failure is devastating, and its mechanisms and mediators are not clear. Tissue injury in one organ appears to trigger disease in remote organs. Kidney and lung are frequently affected, such as when acute kidney injury (AKI) causes acute lung injury (ALI), a frequent clinical condition with high mortality. Here we identify factors secreted from the injured kidney that cause acute lung injury. We developed a murine model mimicking the generation of respiratory failure following acute kidney injury. To identify interorgan crosstalk mediators involved, we performed scRNAseq of mouse kidneys and lungs after AKI. We then applied ligand-receptor (L-R) pairing analysis across cells residing in kidney (ligands) or lung (receptors) to identify kidney-released circulating osteopontin (OPN) as a novel mediator of AKI-induced ALI (AKI-ALI). OPN release very early after AKI largely from tubule cells triggered neutrophil and macrophage infiltration into lungs associated with endothelial leakage, interstitial edema, and functional impairment. Pharmacological or genetic inhibition of OPN prevented AKI-ALI. Transplantation of ischemic wt kidneys into wt mice caused AKI-ALI, while transplantation of ischemic OPN-global-knockout kidneys failed to induce lung endothelial leakage and AKI-ALI, identifying circulating kidney-released OPN as sufficient to cause AKI-ALI in vivo. We show that AKI in humans results in elevations in OPN levels in the serum. Increased serum OPN levels in patients with multiorgan failure have been shown to positively correlate with reduced kidney function, respiratory failure, and mortality. Thus, our results identifying OPN as a mediator of AKI-ALI may have important therapeutic implications in human AKI-ALI and multiorgan failure. |
| Databáze: | OpenAIRE |
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