Determination of Choline in Infant Formula by Ion Chromatography

Autor:	Jeffrey S. Rohrer, Mark Laikhtman
Rok vydání:	1999
Předmět:	Pharmacology Alkaline earth metal Chromatography Conductometry Ion chromatography Hydrochloric acid Conductivity Alkali metal Analytical Chemistry chemistry.chemical_compound chemistry Infant formula Environmental Chemistry Choline Agronomy and Crop Science Food Science
Zdroj:	Journal of AOAC INTERNATIONAL. 82:1156-1162
ISSN:	1944-7922 1060-3271
DOI:	10.1093/jaoac/82.5.1156
Popis:	Choline was determined in infant formula by ion chromatography with suppressed conductivity detection. Samples were digested with 1M hydrochloric acid, filtered, diluted, and injected into the chromatographic system. Choline and the alkali and alkaline earth metals were separated on a high-resolution cation-exchange column and detected by suppressed conductivity. The method was linear between 2 and 200 mg/L (r2 = 0.9999), the concentration range of the diluted samples. This method accurately determined choline in powdered, concentrated, and ready-to-feed infant formulas. Recoveries of choline spikes into powdered infant formula at approximately 1, 0.8, 0.5, and 0.2 times the labeled value ranged from 85 to 114%. This method had good agreement for 8 blind duplicates. The values determined for these samples, which were used in an AOAC collaborative study of an enzymatic method, were consistent with the values determined by the enzymatic method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::edd8f349a8b61e24c911a5dd3ccec4c2 https://doi.org/10.1093/jaoac/82.5.1156 Zobrazit plný text záznamu