Characterization of the Tumor Immune Microenvironment in Soft Tissue Sarcoma Patients Undergoing Surgery
| Autor: | Sylvia M Cruz, Sean J Judge, Morgan A Darrow, Lauren M Perry, Lauren E Farley, Khurshid R Iranpur, Cordelia Dunai, Shuai Chen, Steven W Thorpe, Robert J Canter |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 208:62.05-62.05 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.208.supp.62.05 |
| Popis: | Tumor infiltrating lymphocytes (TILs) have been shown to predict survival in soft tissue sarcomas (STS), but the specific contribution of natural killer (NK) and CD8+ T cells to outcomes is undefined. Therefore, we sought to characterize the extent of NK and CD8+ T cell infiltration in STS. Prospectively, we evaluated 15 patients using fresh tumor from surgery for flow cytometric analysis. Retrospectively, we evaluated archived tumor tissue from 90 STS patients by immunohistochemistry (IHC) for CD3, CD8, CD45RO, NKp46, TIGIT, MHC-I, and p53. We analyzed metastasis-free survival (MFS) and overall survival (OS) by Kaplan-Meier method and log-rank test. By flow cytometry, we observed significant variability in CD45+ leukocytes in the STS TME (mean 29±24% of total live cells) with low percentages of tumor-infiltrating CD3-CD56+ NK cells (1.7±1.9% of total live cells and 5.3±3.0% of live CD45+ cells) and CD8+ T cells (1.6±1.6% of total live cells and 29.6±30.5% of live CD45+ cells). By IHC, NK and T cell infiltrates were low (median H score 0, range 0–66.5 and 2.7, range 0–110, respectively). We confirmed a positive correlation between CD8+ T cell infiltration and significantly improved OS (P Infiltration of NK and CD8+ T cells is overall low in STS patients undergoing surgery but associated with superior OS. Further characterization of the immune infiltrate in STS may yield better biomarkers of prognosis and immune targeting. Supported by the following grants: NIH/NCI 1R03CA252793 NIH/NCI T32 CA251007-01 |
| Databáze: | OpenAIRE |
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