Isolation and Identification of Biosurfactant Producing Bacterial Strain from Saline Soil Samples in Iran; Evaluation of Factors on Biosurfactant Production
| Autor: | Gholamreza Dehghannoudeh, Hamid Forootanfar, Marzieh Sajadi Bami, Mandana Ohadi, Payam Khazaeli |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences biology Chemistry Isolation (microbiology) 16S ribosomal RNA biology.organism_classification 01 natural sciences Agar plate 03 medical and health sciences Bioremediation Microbial enhanced oil recovery Vegetable oil Bacillus atrophaeus 010608 biotechnology Food science General Pharmacology Toxicology and Pharmaceutics Incubation 030304 developmental biology |
| Zdroj: | Jundishapur Journal of Natural Pharmaceutical Products. 15 |
| ISSN: | 2228-7876 1735-7780 |
| DOI: | 10.5812/jjnpp.96798 |
| Popis: | Background: Biosurfactants are a group of valuable amphiphilic molecules that have been widely used in different industries such as pharmaceuticals and cosmetics. Objectives: The current study aimed to screen and identify a bacterial strain able to produce biosurfactant from environmental soil samples, followed by evaluating factors that contribute to biosurfactant production. Methods: The biosurfactants production was evaluated every 24 hours by emulsification index (EI), oil spreading ability, and surface tension measurement. Thereafter, the sequences of 16S rDNA genes and biochemical tests were performed to identify the isolated bacterial. Furthermore, the effect of different factors, including nitrogen, vegetable oil, and cations on biosurfactant productivity of the selected bacterial strain, was also evaluated. Results: In primary screening, out of the six bacterial strains, only one isolate was found to produce a hemolytic zone on the blood agar plate. The EI index was increased in a time-dependent manner with a maximum EI of 10% after 12h. Surface tension measurement revealed decreased surface tension (51 mN/m) after 12 hours' incubation compared to that of the blank sample. The selected isolate was recognized as Bacillus atrophaeus by 16S rDNA gene sequencing and biochemical test. All applied factors in culture media could decrease the surface tension of culture broth compared to that of the blank sample. Conclusions: This study demonstrated a Bacillus strain able to produce biosurfactants. Such biosurfactant producers would have great potentials in bioremediation activities and microbial enhanced oil recovery. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|