RNAzol® RT: a new single-step method for isolation of RNA

Autor:	Amy Kennedy, Piotr Chomczynski, Karol Mackey, Michal Rymaszewski, William W. Wilfinger
Rok vydání:	2010
Předmět:	Small RNA Messenger RNA Chemistry RNA Single step Cell Biology Isolation (microbiology) Biochemistry Single fraction DNA Contamination RNA extraction Molecular Biology Biotechnology
Zdroj:	Nature Methods. 7:4-5
ISSN:	1548-7105 1548-7091
DOI:	10.1038/nmeth.f.315
Popis:	The single-step RNA isolation with RNAzol® RT yields RNA ready for RT-PCR without additional purification or DNase treatment. Previous single-step methods required additional steps to remove DNA contamination before the isolated RNA was used in RT-PCR. RNAzol RT simplifies the single-step method and adds to its versatility and effectiveness. RNAzol RT isolates pure and undegraded RNA with protocols yielding either a single fraction containing total RNA or two separate fractions containing mRNA and small RNA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::edd36da02b28a1868c1e6e41bfa51271 https://doi.org/10.1038/nmeth.f.315 Zobrazit plný text záznamu