Microbial system for identification of antibiotic residues in milk
| Autor: | O. G. Nagel, Rafael Althaus, Pilar Molina |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
0303 health sciences Chromatography 030309 nutrition & dietetics Tetracycline Chemistry 010401 analytical chemistry Oxytetracycline Tylosin 01 natural sciences 0104 chemical sciences Lincomycin 03 medical and health sciences chemistry.chemical_compound Cloxacillin Sulfadiazine medicine Bioassay Ceftiofur Food Science medicine.drug |
| Zdroj: | Journal of Food and Drug Analysis. 19 |
| ISSN: | 2224-6614 |
| DOI: | 10.38212/2224-6614.2185 |
| Popis: | The aim of this study was to evaluate the ResScreen(superscript ®) microbiological system for the identification of antibiotic residues in milk. This microbiological system consists of two methods, the BT (betalactams and tetracyclines) and BS (betalactams and sulfamides) bioassays, containing spores of G. stearothermophilus subsp. calidolactis, culture media and indicators (acid-base and redox). The detection limits of 29 antimicrobial agents were calculated using a logistic regression model.Both methods detect residues of penicillin-G, ampicillin, amoxicillin, cloxacillin, oxacillin, cephalexin, cefoperazone and ceftiofur(superscript ®) at levels close to their Maximum Residue Limits (MRL). The BT bioassay also presents good sensitivity to tetracycline and oxytetracycline residues, whereas the BS bioassay detects sulfadiazine, sulfamethoxazole and sulfathiazole residues in milk.The simultaneous use of both bioassays identifies betalactam, tetracycline and sulfamide residues in milk. Neomycin, tylosin and lincomycin residues can also be detected, but these molecules are positive with the BT and BS bioassays, e.g., betalactams, given the microorganisms' sensitivity to these molecules. |
| Databáze: | OpenAIRE |
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