| Autor: |
Rebeles, J., MacIver, N.J., Nichols, A.G., Beck, M.A., Danzaki, K., Green, W.D., Alwarawrah, Y., Eisner, W. |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| DOI: |
10.17615/0q0q-c141 |
| Popis: |
Background: Obesity is an independent risk factor for increased influenza mortality and is associated with impaired memory T-cell response, resulting in increased risk of infection. In this study, we investigated if weight loss would restore memory T-cell response to influenza. Methods: Male C57BL/6J mice were fed either low-fat or high-fat diet to induce obesity. Once obesity was established, all mice received primary infection with influenza X-31. Following a recovery period, we switched half of the obese group to a low-fat diet to induce weight loss. Fifteen weeks after diet switch, all mice were given a secondary infection with influenza PR8, and memory T-cell function and T-cell metabolism were measured. Results: Following secondary influenza infection, memory T-cell subsets in the lungs of obese mice were decreased compared to lean mice. At the same time, T cells from obese mice were found to have altered cellular metabolism, largely characterized by an increase in oxygen consumption. Neither impaired memory T-cell response nor altered T-cell metabolism was reversed with weight loss. Conclusion: Obesity-associated changes in T-cell metabolism are associated with impaired T-cell response to influenza, and are not reversed with weight loss. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
