Barley tissue as direct template for PCR: a practical breeding tool

Autor:	F. Han, J. A. Clancy, Vadim A. Jitkov, Steven E. Ullrich
Rok vydání:	1996
Předmět:	Pcr cloning food and beverages Plant Science Biology DNA extraction Plant tissue Molecular biology Endosperm law.invention Restriction enzyme Biochemistry law Genotype Genetics Hordeum vulgare Agronomy and Crop Science Molecular Biology Polymerase chain reaction Biotechnology
Zdroj:	Molecular Breeding. 2:181-183
ISSN:	1572-9788 1380-3743
DOI:	10.1007/bf00441433
Popis:	A method for using alkali treated intact plant tissue as a DNA source for the polymerase chain reaction (PCR) was applied to barley. This method saves up to two days and more than USD 50 per 40 samples by eliminating the need for DNA extraction to produce template for PCR. The conditions were optimized for various barley tissues. Fresh leaves, freeze-dried leaves, and anthers worked well as templates while root, embryo, and endosperm tissues did not. The method was shown to work with several genotypes and different primers. The resulting PCR product could be cut with restriction enzyme to produce clear polymorphism without any interference. This method can be a practical breeding tool by providing a fast, inexpensive method for screening large populations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ed88c37806381ac8a4cec72da1a89b7d https://doi.org/10.1007/bf00441433 Zobrazit plný text záznamu Full text from SpringerLink