| Popis: |
The free radical hypothesis has been accepted in the researching of skin aging. As a small molecule oxidant, hydrogen peroxide (H2O2) is easily induce the apoptosis of HSF cells through biofilm system. Oxidative stress caused by H2O2 also leads to the synthesis of inflammatory cytokines, mainly including interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α), which are involved in the regulation of skin inflammation and irritation. Protein protease hydrolysates were prepared from Iris cuttlefish (Symplectoteuthis oualaniensis) and then investigated for their antioxidant activities in vitro. The positive effect by PHCSO (protease hydrolysates from the carcass of Symplectoteuthis oualaniensis) on the reconstruction of HSF (human skin fibroblasts) cells against oxidative stress was investigated by the oxidative stress model via H2O2 inducement. PHCSO-1 or PHCSO-2 was a mixed peptide whose molecular weight is over 10 kDa or less 10 kDa from the ultrafiltration of PHCSO. The reconstructing effect of PHCSO-2 was superior to that of PHCSO on HSF cells according to the results from cell morphology of fluorescence staining, inflammatory factors and antioxidant activity analysis. 5 mg/mL PHCSO-2 showed the DPPH and hydroxyl radical scavenging activity as 57.96% and 56.86% respectively, with TNF-α reduced by 24.83%, which indicated the effective protection on skin from oxidative stress. The protein hydrolysate from Symplectoteuthis oualaniensis was proved to be the marine antioxidant peptide, which could be applied in cosmetics, pharmaceutical or food industries. |
