Callus induction and culture from explants of Erysimum scoparium in a growth regulator-free medium

Autor:	Raquel Martín, Juan Felipe Pérez-Francés, Francisco Valdés
Rok vydání:	1995
Předmět:	Tissue culture Murashige and Skoog medium food.ingredient food Callus Botany Radicle Subculture (biology) Horticulture Biology Cotyledon Hypocotyl Explant culture
Zdroj:	Plant Cell Tissue and Organ Culture (PCTOC). 43:223-228
ISSN:	1573-5044 0167-6857
DOI:	10.1007/bf00039948
Popis:	Calli from cotyledon, hypocotyl and radicle explants of Erysimum scoparium (Brassicaceae) were induced and cultured using a MS medium without growth regulators. Calli obtained from cotyledon or hypocotyl explants showed a higher growth rate while radicle-derived calli exhibited poor growth. Cotyledon-derived calli were compact and green-colored while hypocotyl- and radicle-derived calli were friable and with creamy-green pigmentation. Histological analysis was carried out during culture and the development of abundant vascular elements and meristematic nodules were found. Subculture on a growth regulator-free medium during 10 months did not ecrease callus growth.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ed7a8bfbe7376a4c5b5391fc565351d7 https://doi.org/10.1007/bf00039948 Zobrazit plný text záznamu Full text from SpringerLink