Autor:	Ronald N. Keener, James E. Maneval, Erik J. Fernandez
Rok vydání:	2004
Předmět:	Chromatography Rigidity (electromagnetism) Consolidation (soil) Chemistry Mechanical compression SCALE-UP Modulus Particle size Dimensioning Biotechnology
Zdroj:	Biotechnology Progress. 20:1159-1168
ISSN:	8756-7938
Popis:	We developed and evaluated a model for predicting the flow packing of nonrigid chromatographic resins. The model is based on elasticity theory and accounts for resin rigidity and column diameter. When a modulus determined from a standard mechanical compression (consolidation) test is used, the model captures the primary phenomena of the scale-up process. However, moduli determined from flow-packing experiments improve the accuracy of the predictions and show that the apparent rigidity of chromatographic resins is lower for flow packing than for mechanical compression. Using a modulus from flow-packing experiments provided quantitative scale-up predictions of flow packing carried out in columns with diameters between 200 and 450 mm at different locations and by different operators.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ed7a29694fd29710067f5ea81b91f47b https://doi.org/10.1021/bp0342809 Zobrazit plný text záznamu Plný text