Designing a system of multiplex PCR with hybridization-fluorescent registration of results, on solid substrate, for indication and identification Y. pestis strains
| Autor: | M.A. Makashova, G.A. Eroshenko, V.V. Kutyrev, D.V. Utkin, L.M. Kukleva |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Biotekhnologiya. 36:46-56 |
| ISSN: | 2500-2341 0234-2758 |
| DOI: | 10.21519/0234-2758-2020-36-3-46-56 |
| Popis: | Plague agent strains differ in intraspecific appurtenance, place of origin, as well as their virulence. Development of a rapid and reliable means of Y. pestis strain indication, their identification by intra-specific appurtenance and assessment of their virulence is a relevant issue. To solve the task, we have constructed a system of multiplex PCR with hybridization-fluorescent registration of results on solid substrate, for indication and identification of strains by their belonging to Y. pestis species, sub-species, biovars, phylogenetic lines, also by the presence of the main gene-factors of pathogenicity of chromosomal and plasmid localization. Its effectiveness is confirmed on 114 plague agent strains of various origins. The developed system of multiplex PCR with hybridization-fluorescent registration of results on solid substrate, can be applied for molecular-genetic identification of strains from natural plague foci with specification of their appurtenance to the main phylogenetic branches and assessment of virulence, for enhancement of sanitary-epidemiological control of RF territories and CIS member-states. plague pathogen, strains, indication and identification, PCR |
| Databáze: | OpenAIRE |
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