Abnormal Expression of Trophoblast Major Histocompatibility Complex Class I Antigens in Cloned Bovine Pregnancies Is Associated with a Pronounced Endometrial Lymphocytic Response1
| Autor: | Donald H. Schlafer, Jonathan R. Hill, Christopher J. Davies, Patricia J. Fisher |
|---|---|
| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Biology of Reproduction. 67:55-63 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Early embryonic losses are much higher in nuclear transfer (cloned) pregnancies, and this is a major impediment to improving the efficiency of cloned animal production. In cattle, many of these losses occur around the time of placental attachment from the fourth week of gestation. We studied the potential for altered immunologic status of cloned pregnancies to be a contributing factor to these embryonic losses. Expression of major histocompatibility complex class I (MHC-I) by trophoblast cells and distribution of endometrial T-lymphocyte numbers were investigated. Six 5-wk-old cloned pregnancies were generated, and 2 others at 7 and 9 wk were also included, all derived from the same fetal cell line. All 8 cloned placentas displayed trophoblast MHC-I expression. None of the 8 controls (4–7 wk old) showed any MHC-I expression. The percentage of trophoblast cells expressing MHC-I varied in the clones from 17.9% to 56.5%. Numbers of T lymphocytes (CD3+ lymphocytes) were significantly higher in the en... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|