Augmentation of Soil with Sporangia ofActinoplanesspp. for Biological Control ofPythiumDamping-off
| Autor: | A. B. Filonow, N. I. Khan, L. L. Singleton |
|---|---|
| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Biocontrol Science and Technology. 7:11-22 |
| ISSN: | 1360-0478 0958-3157 |
| Popis: | A method was developed for applying strains of Actinoplanes spp. that are hyper-parasites of oospores of Pythium ultimum to soil for reducing Pythium damping-off of plants. The method is based on the augmentation of soil with sporangia of a strain of Actinoplanes spp. borne on clay granules. In vitro sporulation of strains K30, W57, W257 and 25844 was: (1) greater for most strains on dilute Czapek-Dox agar than on four other agar media; (2) inhibited by continuous exposure to fluorescent light of intensity 4-150 μEm−2s−1, but not by exposure to 1 μEm−2s−1 or darkness; (3) greater at 20-307deg;C than at 10°C;and (4) greater at pH 6-7 than at pH 5 or 8. On solid carriers treated with dilute Czapek-Dox broth (pH 7) and incubated in the dark at 30°C for 3 weeks, strains sporulated poorly or not at all on vermiculite, perlite and rice hulls, but sporulated abundantly (107-109 colony-forming units (CFU) g−1 of granules) on montmorillonite clay granules. When strains 25844, W57 and W257 were applied as granules ... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|