Induction of O-deethylase activity as an index to exposure to coal-derived products and trace environmental pollutants

Autor:	J Kao, W D Bostick, J E Mrochek, J M Holland
Rok vydání:	1981
Předmět:	Oxygenase biology Cytochrome Biochemistry (medical) Clinical Biochemistry Monooxygenase chemistry.chemical_compound chemistry Biochemistry Methylcholanthrene biology.protein Microsome Cytochrome c oxidase Bioassay Energy source
Zdroj:	Clinical Chemistry. 27:1516-1523
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/27.9.1516
Popis:	The metabolism of the synthetic substrate 7-ethoxyresorufin is a selective measure of the activity of cytochrome P-448 monooxygenase, the subset of cytochrome P-450-mediated enzymes preferentially induced by polycyclic aromatic hydrocarbons and related compounds. 7-Ethoxycoumarin metabolism, on the other hand, reflects total (nonselective) cytochrome P-450 monooxygenase activity. Either substrate yields a single, highly fluorescent product, amenable to direct, sensitive assay with the portable centrifugal analyzer. We used three assays with liver microsomes from C57/BL6 mice for the short-term bioassay of the dose-dependent effects of exposure to selected environmental toxins, including petroleums, polychlorinated biphenyls, and their oxidative degradation products.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ed58e17a9b3ab81f89fb7dc58bc625b4 https://doi.org/10.1093/clinchem/27.9.1516 Zobrazit plný text záznamu