Rapid optofluidic detection of biomarkers for traumatic brain injury via surface-enhanced Raman spectroscopy
| Autor: | Valentina Di-Pietro, Jonathan James Stanley Rickard, David J. Smith, Antonio Belli, Pola Goldberg Oppenheimer, David Davies |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Gold layer business.industry Traumatic brain injury Point-of-care testing Biomedical Engineering Medicine (miscellaneous) Bioengineering Surface-enhanced Raman spectroscopy medicine.disease Computer Science Applications 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Clinical decision making Biomarker (medicine) Medicine Spectrum analysis business 030217 neurology & neurosurgery Biotechnology Biomedical engineering |
| Zdroj: | Nature Biomedical Engineering. 4:610-623 |
| ISSN: | 2157-846X |
| Popis: | Current technologies for the point-of-care diagnosis of traumatic brain injury (TBI) lack sensitivity, require specialist handling or involve complicated and costly procedures. Here, we report the development and testing of an optofluidic device for the rapid and label-free detection, via surface-enhanced Raman scattering (SERS), of picomolar concentrations of biomarkers for TBI in biofluids. The SERS-active substrate of the device consists of electrohydrodynamically fabricated submicrometre pillars covered with a plasmon-active nanometric gold layer, integrated in an optofluidic chip. We show that the device can detect N-acetylasparate in finger-prick blood samples from patients with TBI, and that the biomarker is released immediately from the central nervous system after TBI. The simplicity, sensitivity and robustness of SERS-integrated optofluidic technology might eventually help the triaging of TBI patients and assist clinical decision making at point-of-care settings. |
| Databáze: | OpenAIRE |
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