Evidence for biological effects in the radiosensitization of leukemia cell lines by PEGylated gold nanoparticles
| Autor: | Irene Lui, Christopher J. Luby, E. Charles H. Sykes, Benjamin P. Coughlin, Paul T. Lawrence, Charles R. Mace, Daniel J. Spencer |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Cell Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Jurkat cells Flow cytometry medicine General Materials Science medicine.diagnostic_test Cancer General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics medicine.disease Atomic and Molecular Physics and Optics 0104 chemical sciences Leukemia medicine.anatomical_structure Cell culture Colloidal gold Modeling and Simulation Cancer research Nanomedicine 0210 nano-technology |
| Zdroj: | Journal of Nanoparticle Research. 22 |
| ISSN: | 1572-896X 1388-0764 |
| DOI: | 10.1007/s11051-020-4765-1 |
| Popis: | Gold nanoparticles (Au NPs) are promising radiosensitizers for cancer therapy. While the radiosensitizing effects of Au NPs have been shown in many epithelial cancers, there are no documented cases of their use in cells of hematopoietic origin, which constitute ~ 10% of all cancer cases and are frequently treated using radiation therapy. In this work, we measure the uptake of polyethylene glycol-coated (PEGylated) Au NPs (5 nm core diameter) in HL-60 II and Jurkat D1.1 cells using flow cytometry and ICP-AES. Electronic cell counting, metabolic activity assays, and DNA synthesis assays reveal cell line–specific radiosensitization that is independent of the number of internalized nanoparticles. The high SER value for the HL-60 II cell line (1.33 at 5 Gy) points to a dominant biological mechanism. |
| Databáze: | OpenAIRE |
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