Short communication
| Autor: | Peter Chee Tong Chew, Sittampalam Shanmugaratnam, Annamalai Loganath, R. Gunasegaram, K. L. Peh |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
Cholesterol Cholesterol side-chain cleavage enzyme Obstetrics and Gynecology Biology Cleavage (embryo) Umbilical cord In vitro chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Internal medicine Placenta Pediatrics Perinatology and Child Health medicine Pregnenolone Specific activity medicine.drug |
| Zdroj: | jpme. 18:495-499 |
| ISSN: | 1619-3997 0300-5577 |
| DOI: | 10.1515/jpme.1990.18.6.495 |
| Popis: | With a view to establish whether cells of the human umbilical cord possess cholesterol side-chain cleavage activity, homogenates of term umbilical cord obtained following spontaneous vaginal delivery from uncomplicated pregnancies (n = 6; 38-40 weeks gestation) were incubated with 26-14C cholesterol. Controls were homogenates heating in a boiling water bath for 10 min. Proof of the existence of cholesterol C-20, 22-desmolase activity in the viable tissue was established by associating radioactivity due to the labelled carbon 26 of 26-14C cholesterol with the p-bromophenacyl ester of isocaproic acid by reverse-isotope dilution analysis. The desmolase efficiency expressed as specific activity of 14C isocaproic acid, dpm g-1 tissue varied from 110 to 351 with a mean of 202. The small but definite conversion indicates that the cholesterol side-chain cleavage reaction previously believed to be exclusive to the steroidogenic pathway operating in the adrenal, gonads and placenta does exist in the human term umbilical cord in vitro. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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