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A luminescence based biosensor (Rhizotox-C) was used as an indicator of heavy metal pollution of soils. The response of the biosensor to increasing concentrations of total soil Zn, soil solution Zn, soil solution free Zn 2+ , total soil Cu and total soil solution Cu from soils of a long-term sewage sludge field experiment was investigated. The bioluminescence response of the Rhizotox-C biosensor declined as total soil Zn, soil solution Zn and free soil solution Zn 2+ concentrations increased. The EC 25 values for the biosensor for total soil Zn, soluble soil solution Zn and free soil solution Zn 2+ were 164±43 mg kg −1 soil, 4±0.7 and 2±0.3 mg l −1 , respectively. The EC 50 values were 403±57 mg kg −1 soil, 16±3 and 6±1.0 mg l −1 , respectively. The largest soil solution concentration of Cu was about 620 μg l −1 , but this had no significant effect on luminescence. This corresponded to a total Cu concentration in bulk soil of about 349 mg kg −1 . |
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