| Popis: |
To set efficient transformation system in chrysan-themum, thirty-four chrysanthemum ( Dendranthema grandi-florum Kitamura) varieties were collected and cultured for shoot regeneration. Five varieties, ‘Shuho-no-chikara’, ‘Zin-ba’, ‘Baekma’, ‘Pink pride’ and ‘Keumsu’ of them were sele-cted, because they had a high shoot regeneration efficiency. MS medium containing 1.0 mg/L NAA and BA respectively was very adequate for shoot regeneration in those varieties. MS medium with 3.0 mg/L NAA and 1.0 mg/L kinetin in ‘Shuho-no-chikara’ and the medium with 0.5 mg/L NAA and 3.0 mg/L BA in ‘Keumsu’ were also suitable for shoot rege-neration. The most efficient callus induction and shoot rege-neration were obtained on MS medium. Shoot regeneration was enhanced more than 8% on MS medium with 0.3% phy-tagel and 10-15 mg/L putrescine. The best cultural material for shoot regeneration was stem. When stem was used as a cul-ture material, shoot regeneration rate was increased more than 26% and the days to shoot regeneration was shortened about 14 days. |
