Development of a detached leaf procedure to evaluate susceptibility toPlum pox virusinfection by the green peach aphid (Myzus persicae(Sulzer)) in peach

Autor:	Rana Samara, D. T. Lowery, L. W. Stobbs, P. M. Vickers, N. Greig, L.A. Bittner
Rok vydání:	2015
Předmět:	Horticulture Aphid biology Botany food and beverages Pox virus Plant Science Virus multiplication biochemical phenomena metabolism and nutrition Myzus persicae biology.organism_classification Agronomy and Crop Science
Zdroj:	Canadian Journal of Plant Pathology. 37:230-236
ISSN:	1715-2992 0706-0661
DOI:	10.1080/07060661.2015.1035753
Popis:	A method was developed to evaluate susceptibility of peach leaves to Plum pox virus (PPV) infection by aphids. We examined whether virus multiplication could be detected in aphid-inoculated detached leaves and if transmission efficiency of PPV by green peach aphids to detached leaves was comparable with that of peach seedlings. Results demonstrated that transmission efficiencies of viruliferous aphids transferred to detached peach leaves subsequently maintained on an agar layer for 3 weeks was not significantly different from that for intact seedlings. Overlaying infected PPV plum or peach leaf segments on the healthy peach leaves with subsequent application of aphids to the infected leaf pieces provided a comparable transmission efficiency. Reduced handling of the aphids using this method minimized the possibility of damaging the aphids and facilitated higher throughput testing. Comparable infection rates were obtained for detached leaves using either 50 or 25 viruliferous aphids per leaf. Residu...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ed284422f2fbc8f60bb640aeec8972d2 https://doi.org/10.1080/07060661.2015.1035753 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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