How to dissect the Node of Ranvier

Autor: E. W. Park, J. S. Trimmer, M. N. Rasband, D. K. Zhen
Rok vydání: 2008
Předmět:
Zdroj: Journal of Neurochemistry. 81:68-68
ISSN: 1471-4159
0022-3042
DOI: 10.1046/j.1471-4159.81.s1.97.x
Popis: The node of Ranvier is composed of a complex community of axonal and glial proteins responsible for the propagation of electrical signals in myelinated nerve fibers. Both voltage-dependent sodium and potassium channels are found in high densities in discrete axonal membrane domains, but the underlying molecular mechanisms of targeting, clustering and anchoring these proteins are unknown. We have developed monoclonal and polyclonal antibodies against known components of the node of Ranvier and have used these in immunoaffinity chromatographic procedures to co purify proteins associated with these target antigens. The isolated protein fractions were then (1) separated by SDS-PAGE and analyzed by mass-spectrometry and (2) used to immunize mice to develop monoclonal antibodies against novel components of the node of Ranvier. Taking this approach, we have identified a set of candidate interacting proteins that may be important for clustering, targeting, and/or anchoring proteins known to be present at the node of Ranvier. Further, we have developed a library of monoclonal antibodies against novel components of the node of Ranvier, paranode, and juxtaparanode, to be used in expression cloning and mass spectrometry to identify the corresponding antigens.
Databáze: OpenAIRE
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