Automated Immunocytochemistry for Cellular Markers on Cell Smears and Cytospins

Autor: Susan J. Maygarden, Debra B. Novotny, David Cowan, Katherine B. Pryzwansky
Rok vydání: 1992
Předmět:
Zdroj: Journal of Histotechnology. 15:283-288
ISSN: 2046-0236
0147-8885
DOI: 10.1179/his.1992.15.4.283
Popis: A rapid, automated immunocytochemical staining method for the Instrumentation Laboratory Code-On stainer was developed to detect cellular markers in cell smears and cytospins prepared from fine needle aspirates of solid tumors and from body fluids collected by brushing, scraping, or washing. The acetone fixed cell smears and cytospins were stained with monoclonal and polyclonal commercially available antibodies and the Vectastain Elite Mouse andRabbit kits. This system produced reproducible high quality, specific staining of lymphocyte, macrophage, epithelia, and prostatic markers with substantial time and cost savings over manual processing. (The J Histotechnol 15:283, 1992)
Databáze: OpenAIRE