Effect of sterilization processes on the properties of a silane hybrid coating applied to Ti6Al4V alloy
| Autor: | Célia de Fraga Malfatti, Eliena Jonko Birriel, João Antonio Pêgas Henriques, Rosmary Nichele Brandalise, Charlene Silvestrin Celi Garcia, Mariana Roesch Ely, Estela K. Kerstner Baldin |
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| Rok vydání: | 2017 |
| Předmět: |
Materials science
Ethylene oxide Biocompatibility Scanning electron microscope Mechanical Engineering Biomaterial 02 engineering and technology engineering.material Sterilization (microbiology) 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Silane 0104 chemical sciences chemistry.chemical_compound chemistry Coating Mechanics of Materials engineering General Materials Science Fourier transform infrared spectroscopy Composite material 0210 nano-technology |
| Zdroj: | Journal of Materials Research. 33:161-177 |
| ISSN: | 2044-5326 0884-2914 |
| DOI: | 10.1557/jmr.2017.429 |
| Popis: | Sterilization is one of the last stages prior to the implantation of a biomaterial. Therefore, the method should be chosen carefully as this is determinant not to compromise the properties of the material. In this context, three sterilization processes were evaluated as to their effect on the properties of a silane hybrid coating: steam autoclave, ethylene oxide, and hydrogen peroxide plasma. The coating was obtained from a sol consisting of alkoxysilane Tetraethoxysilane and organoalcoxysilane Methyltriethoxysilane (MTES), applied to the Ti6Al4V substrate, to increase its corrosion resistance and biocompatibility. After sterilization, the samples were characterized by scanning electron microscopy, atomic force microscopy, profilometry, wetabillity, and Fourier transform infrared spectroscopy. The electrochemical behavior was monitored by open circuit potential and potentiodynamic polarization curves. The cytocompatibility was evaluated by adhesion, viability, and morphological alterations in the MG-63 cells. The results showed that the protective behavior of the hybrid coating was compromised regardless of the sterilization method. However, the steam autoclave caused more morphological changes on the silane hybrid coating as well as on the Ti6Al4V substrate than the other two sterilization methods. Although the sterilized hybrid coating did not show cytotoxicity, the hybrid coating sterilized by hydrogen peroxide plasma showed a higher percentage of viable cells. The ethylene oxide presented the lowest percentage of viability and the highest cell death rate. |
| Databáze: | OpenAIRE |
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