Chapter 9 Normal Human Prostate Epithelial Cell Cultures

Autor: K. Shankar Narayan, M. Edward Kaighn, Maureen Marnell, Merrill S. Babcock, John F. Lechner
Rok vydání: 1980
Předmět:
DOI: 10.1016/s0091-679x(08)60684-6
Popis: Publisher Summary The chapter discusses the development of procedures to the normal prostate, as either primary explants or fresh tissue. Established culture methods, newly developed techniques, and attention to cellular nutrition have made it possible to isolate reproducibly normal prostatic epithelial cell cultures capable of sustained replication. These cultures are normal epithelial cells based on ultrastructure, karyotype, and inability to grow in soft agar. They were not clonally isolated and are therefore representative of the total neonatal prostatic gland epithelial cell population. The other technique involved replicative epithelial cultures from explanted tissue fragments. This technique is used to initiate prostate cell cultures since 1917. Commonly, epithelial cells migrate from the tissue fragments before the fibroblasts appear. To formulate an effective medium for normal human prostatic epithelial cells, attention must be paid not only to the small-molecular-weight nutrients but to critical growth factors present in fetal bovine serum (FBS).
Databáze: OpenAIRE