Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study
| Autor: | Rania El Majzoub, Michella Ghassibe-Sabbagh, Joseph Sabbagh, Antoine Berberi, Bassam Badran, Mohammad Fayyad-Kazan, Rita Bou Assaf, Fatima Al-Nemer |
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| Rok vydání: | 2021 |
| Předmět: |
030222 orthopedics
Transplantation Mesenchymal stem cell Biomedical Engineering Cell Biology Biology Bone tissue Schneiderian Membrane Regenerative medicine Cell biology Biomaterials 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure stomatognathic system Dental pulp stem cells 030221 ophthalmology & optometry medicine In vitro study Alkaline phosphatase Stem cell |
| Zdroj: | Cell and Tissue Banking. 22:409-417 |
| ISSN: | 1573-6814 1389-9333 |
| DOI: | 10.1007/s10561-020-09887-4 |
| Popis: | Mesenchymal stem cells, being characterized by high self-renewal capacity and multi-lineage differentiation potential, are widely used in regenerative medicine especially for repair of bone defects in patients with poor bone regenerative capacity. In this study, we aimed to compare the osteogenic potential of human maxillary schneiderian sinus membrane (hMSSM)-derived stem cells versus permanent teeth dental pulp stem cells (DPSCs). Both cells types were cultivated in osteogenic and non-osteogenic inductive media. Alkaline phosphatase (ALP) activity assay and quantitative real-time PCR analysis were carried out to assess osteogenic differentiation. We showed that ALP activity and osteoblastic markers transcription levels were more striking in hMSSM-derived stem cells than DPSCs. Our results highlight hMSSM-derived stem cells as a recommended stem cell type for usage during bone tissue regenerative therapy. |
| Databáze: | OpenAIRE |
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