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The purpose of this study was to investigate the aflatoxin B1 binding of lactic acid bacteria (LAB) isolated from Korean traditional soybean paste and to evaluate the effect of incubation conditions and physico-chemical factors on the binding ability of LAB to this mutagen. The amount of aflatoxin B1 bound by Enterococcus faecium DJ22, Lactobacillus fermentum DJ35, Lactobacillus rhamnosus DJ42, and Lactobacillus pentosus DJ47 was strain specific with the percent bound ranging from 19.3% to 52.1%. However, Enterococcus faecalis DJ14, Lactobacillus panis DJ29, and Pediococcus halophilus DJ50 strains did not exhibit any of the binding ability to aflatoxin B1. For most strains, the binding ability was significantly affected by the environmental conditions such as the aflatoxin B1 level, incubation time and temperature, and the initial cell count of LAB. The stability of the aflatoxin B1-bacteria complexes was significantly more unstable after washing. In addition, the binding stability between viable and nonviable cells was not statistically significant. Treatment with heating, acidic pH, α-amylase, protease, lysozyme, or sodium metaperiodate caused a significant (P |
