| Popis: |
Cereals are prone to viral infections and the economic impact of these has increased in recent years. Among these diseases Barley yellow dwarf (BYD) is one of the most destructive diseases of cereals today. For three consecutive years (2014-2015-2016) surveys were carried out in order to search for BYDV species (BYDV-PAV and BYDV-MAV) as well as other cereal viruses (WSSMV, SBMV and BSMV) in seven regions of Algeria (Algiers, Boumerdes, Tipaza Médéa, Adrar, Khenchla and Batna).Targeted samples were taken from plants of different cereal species (wheat, barley, oats). The sample were analyzed by DAS-ELISA and RT-PCR.The results of the ELISA test showed negative responses for the BYDV-MAV, WSSM, SBMV and BSMV viruses and allowed to demonstrate the presence of BYDV-PAV on barley, durum wheat, soft wheat and oats.Thus this viral species was found in all the cereal regions surveyed (North, South, East and West).07 samples (durum wheat, barley and oats) were sequenced and a phylogenetic analyses performed revealed that the Algerian sequences clustered in group I and group II. |
