Simultaneous Determination of Ibuprofen and Diphenhydramine Citrate in Tablets by Validated LC

Autor: Dantu Durga Rao, K. Mukkanti, P. Venkat Rao, Dinesh Chakole, Shakil S. Sait
Rok vydání: 2009
Předmět:
Zdroj: Chromatographia. 69:1133-1136
ISSN: 1612-1112
0009-5893
DOI: 10.1365/s10337-009-0977-3
Popis: A stability-indicating LC method was developed for the simultaneous determination of ibuprofen and diphenhydramine citrate in pharmaceutical dosage forms. The chromatographic separation was achieved on an Inertsil ODS 3V, 150 × 4.6 mm, 5 μm, column. The mobile phase contained a mixture of 50 mM potassium dihydrogen phosphate buffer:acetonitrile:triethylamine:glacial acetic acid (55:45:0.2:0.2, v/v/v/v). This method allowed the determination of 2.85–9.14 mg mL−1 of ibuprofen and 0.54–1.73 mg mL−1 of diphenhydramine citrate, in a diluent consisting of pH 7.2, 50 mM potassium dihydrogen phosphate buffer:acetonitrile (40:60, v/v). The flow rate was 1.2 mL min−1 and the detection wavelength was 260 nm. The limit of detection for ibuprofen and diphenhydramine citrate was 1.72 and 0.54 μg mL−1 and the limit of quantification was 5.73 and 1.64 μg mL−1, respectively. This method was validated for accuracy, precision and linearity. The method was also found to be stability indicating.
Databáze: OpenAIRE
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