Gut microbiota assessment in Moscow long-livers using next generation sequencing
| Autor: | EN Ilina, Denis A Gudkov, Olga N. Tkacheva, Irina D. Strazhesko, Oksana E. Glushchenko, Elizaveta V Starikova, Natalia Klimenko, Daria A. Kashtanova |
|---|---|
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | MicroRNA in ocular pathology. :16-20 |
| ISSN: | 2542-1204 2500-1094 |
| Popis: | Demographic aging poses a challenge to the medical community, pressing for research into the biological factors promoting longevity and its features. Below, we look at the gut microbiota as one of such factors. The aim of this non-longitudinal study was to profile the gut microbiota of centenarians and to compare it with that of relatively healthy, younger Moscow residents. The study recruited 20 people aged 97–100 years (mean age 98 ± 1 year); the control group consisted of 92 individuals aged 53 ± 13 years. For each stool sample, the variable V3–V4 regions of the microbial 16S rRNA gene were sequenced. Primary analysis, read filtering and taxonomic identification were conducted in the QIIME 1.9 environment; reconstruction of metabolic pathways was aided by PICRUSt. Statistical analysis was performed by means of Python v. 3.2. A few differences were detected between the gut microbiota of centenarians and younger individuals: Bifidobacterium (p = 0.026) and Coprococcus eutactus (р = 0.026) were more abundant in centenarians, whereas Bacteroides (p = 0.003) and Prevotella (р = 0.002) were better represented in younger participants. The potential for butyric acid synthesis was higher in the group of centenarians (p = 0.048). Surprisingly, the gut microbiota of centenarians was more diverse and surprisingly beneficial for advanced age. Besides, the gut microbiota of centenarians might have more pronounced anti-inflammatory potential due to its ability to better synthesize butyric acid. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|