Problems in the size exclusion chromatography of poly(N-isopropylacrylamide) on styragel columns
| Autor: | E. O. Perepelitsina, Ya. I. Estrin, A. A. Grishchuk |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
Sorbent Chromatography Elution Size-exclusion chromatography 02 engineering and technology Polymer 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences chemistry.chemical_compound Column chromatography chemistry Poly(N-isopropylacrylamide) Polystyrene Physical and Theoretical Chemistry 0210 nano-technology Tetrahydrofuran |
| Zdroj: | Russian Journal of Physical Chemistry A. 90:1474-1478 |
| ISSN: | 1531-863X 0036-0244 |
| DOI: | 10.1134/s0036024416070086 |
| Popis: | The molecular weights of poly(N-isopropylacrylamide) (PNIPA), calculated according to polystyrene calibration standards upon the elution of THF on styragel columns, appear to be much lower than their actual values determined using independent approaches. This is likely due to interactions between the nitrogen-containing units of PNIPA polymer chains and the sorbent, so the polymer is eluted in the mode intermediate between exclusion and critical. An effective exclusion mode during the elution of PNIPA on a styragel column can be achieved by using an eluent more polar than tetrahydrofuran (particularly, 1-methylpyrrolidone). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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