Effects of Clomazone on IPP Isomerase and Prenyl Transferase Activities in Cell Suspension Cultures and Cotyledons of Solanaceous Species
| Autor: | Kathleen M. Hanley, Jon E. Scott, Leslie A. Weston, Joseph Chappell |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification biology Nicotiana tabacum fungi food and beverages 04 agricultural and veterinary sciences Plant Science biology.organism_classification 01 natural sciences Lycopersicon In vitro Enzyme assay 010602 entomology chemistry.chemical_compound Horticulture chemistry Chlorophyll Pepper 040103 agronomy & agriculture biology.protein 0401 agriculture forestry and fisheries Clomazone Agronomy and Crop Science Carotenoid |
| Zdroj: | Weed Science. 42:509-516 |
| ISSN: | 1550-2759 0043-1745 |
| Popis: | Laboratory assays were conducted to determine the sensitivity of tomato and tobacco cell suspension cultures and tomato and pepper cotyledons to clomazone. A comparison of fresh weight and carotenoid content indicated up to a three-fold difference between the clomazone-tolerant to- bacco and clomazone-susceptible tomato cell suspension cul- tures. In contrast, an approximate 60-fold difference between the tolerant pepper and susceptible tomato cotyledons was observed when total chlorophyll and carotenoid contents were measured. The effect of clomazone and its possible metabolites on in vivo and in vitro extractable IPPisomerase (EC 53.3.2) and prenyl transferase (EC 2.5.1.29) activity was investigated. There was no clear inhibitory effect of cloma- zone or possible clomazone metabolites upon enzyme activity in tomato or tobacco cell suspension cultures or on light or dark grown tomato or pepper cotyledons. No specific enzy- matic target site of clomazone was identified in correlation with the reduction in total chlorophyll or carotenoid content. Nomenclature: Clomazone, 2-((2-chlorophenyl)methyl)-4,4- dimethyl-3-isoxazolidinone; pepper, Capsicum annum L. cv Yolo Wonder L; tobacco, Nicotiana tabacum L. 'KY-14'; tomato, Lycopersicon esculentum Mill. 'Niagara.' |
| Databáze: | OpenAIRE |
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