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BACKGROUND: Soybean seeds are rich in natural protein and are favorable environments for targeted protein expression. Soybeans represent an ideal platform for the production of novel vaccines that, in theory, do not require a cold chain. This studyinvestigatedthestabilityofasoybean-derivedantigenfollowinglong-termstorage,formulation,andshipmentoverseas in the absence of refrigeration. RESULTS:Transgenicseedscanbestoredformorethan4 yearsatambienttemperaturewithnodetectablelossofFanCantigen stability. Conventional processing methods utilizing heat, mechanical extraction and solvent extraction resulted in practical formulations that could be lyophilized, stored as dried milk powder and rehydrated in water without loss of FanC antigen stability.Overseasshipmentoftransgenicseedpowderandsoymilkformulationsintheabsenceofacoldchainhadnoadverse effects on formulations or the FanC antigen. CONCLUSION:Thefeasibilityoflong-termstorage,processingandshipmentoftransgenicsoyproductsintheabsenceofacold chain was demonstrated. Soybeans represent a practical platform for development of novel vaccines to potentially address the worldwide need for vaccines that are cost-effective, easy to formulate and can be manufactured, stored and transported without refrigeration. c � 2009 Society of Chemical Industry |
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