Evaluation of a Panel of Tumor Markers for Molecular Detection of Circulating Cancer Cells in Women with Suspected Breast Cancer

Autor:	Andrea Nibbe, Kathleen A Kitzmann, Vera J. Suman, Barbara K. Zehentner, Wilma L. Lingle, Patrick C. Roche, Raymond L. Houghton, Monica M. Reinholz, Leslie M. Jonart, Janles N. Ingle
Rok vydání:	2005
Předmět:	Oncology Breast biopsy Cancer Research medicine.medical_specialty Pathology biology medicine.diagnostic_test business.industry Cancer Ductal carcinoma medicine.disease Mammaglobin Breast cancer Circulating tumor cell Internal medicine Cancer cell medicine biology.protein Breast disease business
Zdroj:	Clinical Cancer Research. 11:3722-3732
ISSN:	1557-3265 1078-0432
DOI:	10.1158/1078-0432.ccr-04-1483
Popis:	Purpose: We examined the feasibility of using molecular characterization of circulating tumor cells as a method for early detection of breast cancer. Research Design: Women without a prior history of cancer who had a breast abnormality detected on imaging followed by a breast biopsy were enrolled in this study. Density gradient centrifugation and immunomagnetic capture were used to enrich for epithelial cells from ∼20 mL of blood. Real-time reverse transcription-PCR was used to quantitate the expression levels of the highly breast-specific genes, mammaglobin, γ-aminobutyric acid type A receptor π subunit (GABA Aπ), B305D-C, and B726P in the epithelial cell–enriched samples. Results: The assay was technically feasible in 154 of 199 accrued patients. From their clinical assessment, 100 patients had benign breast disease, 10 patients had ductal carcinoma in situ, and 44 patients had invasive breast cancer. We constructed a diagnostic test that classified patients with mammaglobin levels of at least 32.2 copies/pg β-actin (units) in their circulating epithelial cells as positive for invasive breast cancer. This resulted in a sensitivity and specificity of 63.3% and 75.0%, respectively. A diagnostic test that classified patients as positive for invasive breast cancer when either mammaglobin levels were >46.3 units or B305D-C levels were >11.6 units increased the sensitivity and specificity to 70.5% and 81.0%, respectively. In the latter test, 12 of the 14 node-positive breast cancer patients were correctly identified. Including GABA Aπ and B726P in the test did not increase its diagnostic potential. Conclusions: These results suggest that molecular characterization of circulating epithelial cells using mammaglobin and B305D-C offers potential for early detection of invasive breast cancer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ecb0cad5abe0174eb63e6a277116992a https://doi.org/10.1158/1078-0432.ccr-04-1483 Zobrazit plný text záznamu