| Autor: |
Abraham Zelkowicz, David Fanberstein, Oren Tirosh, Sharon Farfouri, Ron Kohen |
| Rok vydání: |
1999 |
| Předmět: |
|
| DOI: |
10.1016/s0076-6879(99)00148-2 |
| Popis: |
Publisher Summary As a major contribution to the antioxidant activity is derived from low molecular weight antioxidants (LMWA) that can easily penetrate via cell membranes and provide local protection, a procedure has been developed for evaluating antioxidant activity contributed by LMWA that are present on the surface of the skin and can be secreted from deeper layers to the outer environment. This chapter describes method suitable for both indirect and direct evaluation of skin antioxidant activity. The method is also suitable for evaluating lipid hydroperoxide levels on the surface of the skin as an indicator of oxidation status of the skin. Quantification of reducing antioxidants can be achieved because they are secreted from the skin surface into a well containing an extraction solution. Analysis of the reducing equivalents released indicates the presence of uric acid and ascorbic acid. Direct measurement allows continuous determination of the release of LMWA and their interaction with the iron chelate. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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